Publications

Authors:

Zoico, Elena; Garbin, U; Olioso, Debora; Mazzali, G; FRATTA PASINI, Anna Maria; DI FRANCESCO, Vincenzo; Sepe, A; Cominacini, Luciano; Zamboni, Mauro

Title:

The effects of adiponectin on interleukin-6 and MCP-1 secretion in lipopolysaccharide-treated 3T3-L1 adipocytes: role of the NF-kappaB pathway.

Year:

2009

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

A Stampa

Referee:

Sì

Name of journal:

International Journal of Molecular Medicine

ISSN of journal:

1107-3756

N° Volume:

24

Number or Folder:

6

Page numbers:

847-851

Keyword:

adiponectin; IL-6; adipocyte

Short description of contents:

It was recently suggested that the transcription nuclear factor-kappaB (NF-kappaB) plays an important role in controlling the inflammation and metabolic alterations associated with obesity. In endothelial and monocytic cells, adiponectin acts as a modulator of the inflammatory response, suppressing NF-kappaB activation. The aim of this study was to assess the ability of different forms of adiponectin to modulate the inflammatory response in adipocytes. 3T3-L1 preadipocytes were cultured according to standard conditions. Fully differentiated adipocytes were stimulated with 1 microg/ml lipopolysaccharides (LPS) for 16 h, with or without pre-treatment with 10 microg/ml of globular (AdG) or full-length (AdFl) adiponectin. Both AdG and AdFl significantly suppressed LPS-induced expression of IL-6 mRNA in adipocytes and reduced the concentration of IL-6 in culture media. Adiponectin pre-treatment significantly reduced the increase in MCP-1 mRNA in adipocytes exposed to LPS. In culture media, the increase in MCP-1 detected after LPS stimulation was significantly attenuated after pre-treatment with AdG. In 3T3-L1, AdG and AdFl reduced NF-kappaB activity by 50 and 40%, respectively compared to the NF-kappaB activation induced by LPS alone. Moreover, both forms of adiponectin significantly attenuated IkappaB-alpha as well as IKK gene expression. Pre-treatment of adipocytes with AdG or AdFl significantly increased PPARgamma mRNA levels, taking its expression back to the basal level. Both AdG and AdFl exert anti-inflammatory activity suppressing IL-6 and MCP-1 production from inflamed adipocytes. This anti-inflammatory action may be mediated through inhibition of NF-kappaB activity as well as through increased PPARgamma expression.

Product ID:

56295

Handle IRIS:

11562/338138

Deposited On:

May 16, 2010

Last Modified:

March 8, 2024

Bibliographic citation:

Zoico, Elena; Garbin, U; Olioso, Debora; Mazzali, G; FRATTA PASINI, Anna Maria; DI FRANCESCO, Vincenzo; Sepe, A; Cominacini, Luciano; Zamboni, Mauro, The effects of adiponectin on interleukin-6 and MCP-1 secretion in lipopolysaccharide-treated 3T3-L1 adipocytes: role of the NF-kappaB pathway. «International Journal of Molecular Medicine» , vol. 24 , n. 6 , 2009 , pp. 847-851

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